Electrochemical Studies Of Hydydrolytic Products Of Palm Oil

This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. The released fatty acids were used as a substrate for lipoxygenase immobilized on modified Nafion membrane carbon electrode and the current generated was studied by cyclic voltammetry. The electrooxidation of glycerol (obtained at optimum conditions of hydrolysis of palm oil) in the presence of nicotinamide adenine dinucleotide was studied using glycerol dehydrogenase enzyme immobilized in ammonium modified Nafion membrane on polymer modified carbon cloth electrodes.Due to the large overvoltage encountered for NADH oxidation at the electrode, five conducting polymers viz. polymethylene green , polyaniline, poly(ortho-phenylene diamine), poly(4-vinyl pyridine), and polypyrrole were used to regenerate NAD+ and to shuttle electrons from the NADH to the electrode. The electrochemical oxidation of glycerol was also studied by another two enzymes system, monoenzyme system, alcohol dehydrogenase and a bienzyme system of alcohol dehydrogenase and aldehyde dehydrogena.